Download Current Protocols in Chemical Biology 2010 (Volume 2) by Adam P. Arkin PDF

By Adam P. Arkin

ISBN-10: 0470559276

ISBN-13: 9780470559277

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Overview of peptide and protein analysis by mass spectrometry. Curr. Protoc. Protein Sci. 27. 36 Volume 2 Current Protocols in Chemical Biology Construction and Use of Glycan Microarrays Christopher T. Campbell,1 Yalong Zhang,1 and Jeffrey C. Gildersleeve1 1 National Cancer Institute, Frederick, Maryland ABSTRACT Glycosylation is an important post-translational modification that influences many biological processes critical for development, normal physiologic function, and diseases. Unfortunately, progress toward understanding the roles of glycans in biology has been slow due to the challenges of studying glycans and the proteins that interact with them.

Com). ch090228 Copyright C 2010 John Wiley & Sons, Inc. 37 Volume 2 Neoglycoproteins Acquisition of a diverse set of glycans and carbohydrates is a key step for construction of a glycan array. The focus of this protocol is on the production of neoglycoprotein arrays. Neoglycoproteins are non-naturally-occurring conjugates formed by covalently linking a glycan to a carrier protein, such as albumin. , 2008). Using commercial sources and simple conjugations, one can obtain approximately 50 to 100 neoglycoproteins at present.

Keep a record of any defects, and save an electronic image of the arrays, if possible. 45 mm 12 4 mm/sec 0 sec 1 2 Number of wash cycles Number of times the entire wash cycle is repeated 2 Glycan Microarrays 41 Current Protocols in Chemical Biology Volume 2 clean pin debris in channel debris on pin surface Figure 2 Inspection of pins prior to printing. Magnified views of the pins are shown. The full length of the pin should be inspected for any debris that may clog the channel. A clean pin is free of debris throughout its channel and near the pin tip.

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Current Protocols in Chemical Biology 2010 (Volume 2) by Adam P. Arkin


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