By Lynne S. Garcia
This 3rd variation includes collaborative efforts of over a hundred and fifty skilled medical microbiologists, clinical laboratory technologists, and laboratory supervisors. This well-respected reference maintains to function the only significant ebook delivering step by step protocols and outlines that allow scientific microbiologists and laboratory employees group of workers to accomplish all analyses, together with acceptable quality controls options, from the receipt of the specimen via processing, checking out, interpretation, presentation of the ultimate record, and next session. to deal with the increasing position of medical microbiologists, the recent variation locations higher emphasis on components resembling molecular techniques, bioterrorism, safeguard, and epidemiology/infection keep watch over in scientific amenities. approaches are formatted to stick to the GP02-5A (2006) record of the nationwide Committee for medical Laboratory Standards/Clinical and Laboratory criteria Institute (NCCLS/CLSI). The icons within the margin of the textual content relate to safeguard concepts, using ordinary precautions, a reminder for the person to list correct reagent dates (receipt, opened, and expiration), in addition to to augment quality controls.
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desk of Contents
Volume 1 1. approach Coding, repayment, and Billing Compliance
2. Specimen assortment, delivery, and Acceptability
three. cardio Bacteriology
four. Anaerobic Bacteriology
Volume 2 five. Antimicrobial Susceptibility trying out
6. cardio Actinomycetes
7. Mycobacteriology and Antimycobacterial Susceptibility trying out
eight. Mycology and Antifungal Susceptibility checking out
Volume three 10. Viruses and Chlamydiae
12. Molecular Diagnostics
thirteen. Epidemiologic and an infection regulate Microbiology
14. caliber insurance, qc, Laboratory files, and Water caliber
15. Biohazards and defense
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Extra resources for Clinical Microbiology Procedures Handbook, Volumes 1-3
Uncontaminated fluid Sterile tube Premature rupture of membranes >24 h Sterile container with TM; appropriate collection containers for demonstration of specific organisms, such as N. gonorrhoeae, Chlamydia, HSV, etc. 1-1). Venereal disease, postpartum infection Genital tractfemale Amniotic fluid Cervix (endocervix) Wipe cervix clean of vaginal secretion and mucus. Use speculum and no lubricant. Uncontaminated endocervical secretions; take two swabs. Cul de sac (culdocentesis) Surgical procedure Fluid, secretions Container Venereal disease; pelvic inflammatory disease Comments Treat as any other normally sterile body fluid; may contain Neisseria gonorrhoeae.
May require culture for mycobacteria, legionellae in addition to DFA, stains for Pneurnocystis, culture for fungi, viruses, and parasites. Culture processing must be preceded by Gram stain demonstrating >25 PMNs and < 10 epithelial cells/lpf. See section 7 on acid-fast organisms if submitted for mycobacteria. May be refrigerated overnight. Cellular composition may be misleading due to inflammatory reaction caused by endotracheal tube. May be refrigerated. Process immediately. ” Consider quantitative culture.
See “Anaerobic cultures” for aspirate. Slide and coverslip or capillary tube History of discharge Comments Likelihood of external contamination is high for cultures obtained through the vagina. , Actinomyces, Torulopsis, and other yeasts. May require sending to reference laboratory. Occasionally, this type of specimen is expelled into toilet and is grossly contaminated. Discharge may be stimulated by gently stripping and massaging the urethra against public symphysis through the vagina. Consider venereal, fungal, anaerobic, and AFB infection.
Clinical Microbiology Procedures Handbook, Volumes 1-3 by Lynne S. Garcia