Download Advances in Virus Research by Kenneth M. Smith and Max A. Lauffer (Eds.) PDF

By Kenneth M. Smith and Max A. Lauffer (Eds.)

ISBN-10: 0120398117

ISBN-13: 9780120398119

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He assumed that a single mutant was prcsent in the inoculum applied to n plant, if that plant showed secondary symptoms on noninoculatcd leaves, This type of analysis leads to an crror because 2 or more particles capable of initiating systemic infection may have been applied to some plants. The magnitude of this error can be estimated if the reasonable assumption is made that the variants are randomly distributed among the inoculated plants. In this instance, however, the error is impossible to assess because of the paucity of experimental detail.

25 26 26 27 28 29 31 31 33 42 43 44 45 46 47 55 58 I. INTRODUCTION One of the outstanding problems of biology is the nature of the relationship between gene and gene product. Viruses offer a useful material for attacking this problem because virus particles consist of a small number of genes and one to several gene products. The smalI ribonucleic acid-containing viruses are particularly useful for this purpose because many of them contain only enough nucleic acid to comprise a small number of genes and only a single species of protein as a gene product.

Iv. The total number of mutants induced by nitrous acid, treatment. Of the several properties of TMV that have been examined, the two that are most frequeiitly altered are symptom expression in a systemic host and the size of necrotic local lesions in a local lesion host (Siegel, 40 ALBERT SIEGEL 1960). When survivors of nitrous acid-treated virus are screened for alteration in both of thcse properties, it is found that 1 mutating deamination occiirs for each 2 to 3 killing deaminations. Schuster and Schramm (1958) calculated that a deamination is lethal if it occurs to any one of about 3000 of the base residues contained in an infectious nucleic acid molecule, and there is reason to believe that this number is similar when the intact virus is treated.

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Advances in Virus Research by Kenneth M. Smith and Max A. Lauffer (Eds.)

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